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Related Concept Videos

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Related Experiment Video

Updated: Nov 25, 2025

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
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Gene expression network analysis provides potential targets against SARS-CoV-2.

Ana I Hernández Cordero1, Xuan Li2, Chen Xi Yang2

  • 1Centre for Heart Lung Innovation, University of British Columbia, Vancouver, BC, Canada. Ana.Hernandez@hli.ubc.ca.

Scientific Reports
|December 15, 2020
PubMed
Summary

Researchers identified genes co-expressed with SARS-CoV-2 entry factors, angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2). Many co-expressed genes are druggable targets, potentially accelerating COVID-19 therapeutic development.

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Area of Science:

  • Genomics
  • Virology
  • Pharmacology

Background:

  • Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) utilizes host cell angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) for cellular entry.
  • Understanding host-pathogen interactions is crucial for developing effective COVID-19 treatments.

Purpose of the Study:

  • To identify and characterize genes co-expressed with ACE2 and TMPRSS2 in lung tissue.
  • To explore the biological functions of these co-expressed genes.
  • To identify potential druggable targets among these genes for COVID-19 therapeutics.

Main Methods:

  • Weighted Gene Correlation Network Analysis (WGCNA) was applied to gene expression profiles from 1,038 lung tissue samples.
  • Bioinformatics databases were used to explore gene functions and known drug-gene interactions.
  • Gene Ontology enrichment analysis was performed for TMPRSS2-correlated genes.

Main Results:

  • ACE2 was part of a module with 681 co-expressed genes; 10 showed moderate-high correlation with ACE2 and had known drug interactions.
  • TMPRSS2 was in a module of 1,086 co-expressed genes, with 31 enriched in 'receptor-mediated endocytosis'.
  • 52 TMPRSS2-correlated genes had known interactions with existing drug compounds.

Conclusions:

  • Dozens of genes are co-expressed with ACE2 and TMPRSS2, suggesting potential roles in COVID-19 pathophysiology.
  • Many identified co-expressed genes are potentially targetable with existing drugs.
  • This finding may expedite the development of novel therapeutics for COVID-19.