Untargeted metabolite profiling of liver in mice exposed to 2-methylfuran

Furan, a significant food contaminant, was found in many cooked foods. In most cooked foods, furan has been found to be coexisted with some alkylated derivatives such as 2-methylfuran. 2-methylfuran was found to be potent hepatotoxins. Little toxicological data is available for 2-methylfuran. The objective of this study was to investigate metabolite changes in the liver samples from mice fed with 2-methylfuran by untargeted metabolomic approach. Metabolomic analysis was conducted by using gas chromatography coupled with mass spectrometry (GC-MS). Twenty-four metabolites were identified as differential metabolites. The important metabolic pathway was linoleic acid metabolism, glycine, serine, and threonine metabolism, methane metabolism, ascorbate, and aldarate metabolism, valine, leucine, and isoleucine biosynthesis, arachidonic acid metabolism, alanine, aspartate, and glutamate metabolism, aminoacyl-tRNA biosynthesis, cysteine, and methionine metabolism, inositol phosphate metabolism, and pyruvate metabolism. These newly identified pathways provide evidence for investigating toxic mechanism of 2-methylfuran. PRACTICAL APPLICATION: Furan in foods has caused public health concern for its hepatotoxicity and hepatic carcinogenicity in rodents. The metabolomics method was constructed to find more biomarkers to study underlying hepatotoxic mechanisms of 2-methylfuran. It will offer important information for official limits of 2-methylfuran in foods.