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Updated: Nov 25, 2025

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

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Published on: December 8, 2023

Efficient Portable Urea Biosensor Based on Urease Immobilized Membrane for Monitoring of Physiological Fluids.

JeeYoung Kim^1,2, Gun Yong Sung^1,2,3, Min Park^1,2,3

¹Cooperative Course of Nano-Medical Device Engineering, Hallym University, Chuncheon, Gangwon-do 24252, Korea.

|December 16, 2020

Summary

This summary is machine-generated.

A novel urea biosensor utilizes disuccinimidyl suberate (DSS) for efficient urease immobilization, outperforming glutaraldehyde (GA) for real-time physiological fluid monitoring.

Keywords:

disuccinimidyl cross-linker flow system real-time monitoring urea biosensor urease immobilization

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Area of Science:

Biomedical Engineering
Biosensor Technology
Biomaterial Science

Background:

Glutaraldehyde (GA) is a common cross-linker but suffers from polymerization issues.
Urease immobilization is crucial for urea biosensor development.
Real-time monitoring of physiological fluids requires stable and efficient biosensors.

Purpose of the Study:

To develop a portable urea biosensor for real-time monitoring of physiological fluids.
To investigate the efficacy of disuccinimidyl cross-linkers for urease immobilization.
To compare the performance of the developed biosensor with glutaraldehyde-based systems.

Main Methods:

Urease was immobilized on polytetrafluoroethylene (PTFE) using disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS), and bis-N-succinimidyl-(pentaethylene glycol) ester (BS(PEG)5).
Urease activity and immobilization were confirmed using activity tests and FT-IR analysis.
The biosensor was integrated into a polydimethylsiloxane (PDMS) fluidic chamber for chronoamperometric measurements of flowing urea samples.

Main Results:

Disuccinimidyl suberate (DSS) demonstrated the highest urease immobilizing efficiency.
The DSS urea biosensor exhibited superior sensitivity compared to glutaraldehyde (GA).
The biosensor showed suitable repeatability and interference resistance for monitoring urea in human serum.

Conclusions:

Disuccinimidyl suberate (DSS) is an effective cross-linker for urease immobilization in urea biosensors.
The developed portable urea biosensor offers a promising alternative for real-time physiological fluid analysis.
This technology has potential applications in clinical diagnostics and patient monitoring.