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Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Nov 25, 2025

Evaluating Regional Pulmonary Deposition using Patient-Specific 3D Printed Lung Models

Evaluating Regional Pulmonary Deposition using Patient-Specific 3D Printed Lung Models

Published on: November 11, 2020

Regional Deposition: Targeting.

Andrew R Martin¹

¹10-324 Donadeo Innovation Center for Engineering, University of Alberta, Alberta, Canada.

Journal of Aerosol Medicine and Pulmonary Drug Delivery

|December 16, 2020

Summary

This summary is machine-generated.

Targeting aerosol deposition in the lungs can be achieved by manipulating particle size and inhalation. Advanced techniques aim for precise regional lung targeting, crucial for future aerosol medicine applications.

Keywords:

aerodynamic size central deposition deposition pattern inhalation drug delivery inhalation pattern particle size peripheral deposition targeted drug delivery

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Area of Science:

Pulmonary drug delivery
Aerosol science
Respiratory medicine

Background:

Regional aerosol deposition in the lungs is influenced by particle size and inhalation patterns.
Targeted deposition aims to control where aerosols deposit in the respiratory tract for therapeutic benefit.

Purpose of the Study:

To review existing evidence for targeted aerosol deposition.
To introduce various methods for achieving regional and local lung targeting.
To discuss the future research directions and applications of precise aerosol deposition.

Main Methods:

Review of existing literature on aerosol deposition and targeting strategies.
Categorization of targeting approaches into passive (regional) and active (local).
Discussion of factors influencing deposition: particle size, inhalation, hygroscopic changes, carrier gases (e.g., helium/oxygen).

Main Results:

Passive targeting to central or peripheral lung regions can be achieved through inhalation timing, aerosol properties, and gas mixtures.
Local, spatially-defined lung targeting is an emerging field with early-stage techniques.
The precision required for therapeutic benefit remains an open research question.

Conclusions:

Current methods allow for regional targeting of aerosol deposition in the lungs.
Highly precise, local targeting is still in its early developmental stages.
Further research is needed to determine optimal targeting precision and identify applications benefiting from selective deposition.