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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

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Related Experiment Video

Updated: Nov 25, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

AnnoLnc: A One-Stop Portal to Systematically Annotate Novel Human Long Noncoding RNAs.

De-Chang Yang^1,2, Lan Ke^1,2, Yang Ding^1,2

¹Biomedical Pioneering Innovation Center (BIOPIC), Beijing Advanced Innovation Center for Genomics (ICG), Center for Bioinformatics (CBI), Peking University, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)

|December 16, 2020

Summary

AnnoLnc is a comprehensive online portal for annotating human long noncoding RNAs (lncRNAs). It provides systematic annotation of lncRNA functions and regulation using over 700 data sources.

Keywords:

Annotation Long noncoding RNAs Web server

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Published on: July 22, 2016

Area of Science:

Genomics
Bioinformatics
Molecular Biology

Background:

Over 100,000 long noncoding RNAs (lncRNAs) are identified in the human genome.
The biological functions and regulatory mechanisms of most lncRNAs remain largely unknown.

Purpose of the Study:

To present AnnoLnc, a comprehensive online annotation portal for human lncRNAs.
To provide on-the-fly annotation for novel human lncRNAs using extensive data sources.

Main Methods:

Development of AnnoLnc, a web server and API for lncRNA annotation.
Integration of over 700 data sources for systematic lncRNA analysis.
Annotation includes genomic location, secondary structure, expression, coexpression, regulation, interactions, and evolution.

Main Results:

AnnoLnc offers a user-friendly web interface and integrates with existing pipelines via web service APIs or a stand-alone version.
It systematically annotates inputted lncRNAs across multiple biological dimensions.
Provides comprehensive functional and regulatory insights for novel lncRNAs.

Conclusions:

AnnoLnc serves as a valuable resource for researchers studying human lncRNAs.
Facilitates the exploration of lncRNA functions, regulation, and biological roles.
Enhances the understanding of the lncRNA landscape in the human genome.