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Liquid Biopsies in Ewing Sarcoma.

Manuela Krumbholz1, Markus Metzler2

  • 1Department of Pediatrics, University Hospital Erlangen, Erlangen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|December 16, 2020
PubMed
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Liquid biopsies offer noninvasive monitoring for solid tumors. A new method uses genomic fusion sequences as Ewing sarcoma liquid biopsy markers when other serum markers are absent.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Diagnostics

Background:

  • Liquid biopsies allow noninvasive monitoring of solid tumor therapies.
  • Serum markers like proteins and enzymes can quantify tumor burden.
  • Many pediatric tumors lack suitable serum markers, necessitating alternative approaches.

Purpose of the Study:

  • To develop a novel liquid biopsy method for Ewing sarcoma.
  • To utilize tumor-specific genetic alterations as noninvasive biomarkers.
  • To overcome limitations of traditional serum marker-based monitoring.

Main Methods:

  • Developing a method to detect genomic fusion sequences in patient samples.
  • Utilizing these fusion sequences as biomarkers for Ewing sarcoma.
  • Applying this technique for noninvasive therapy monitoring.
Keywords:
Cell-free circulating DNADouble-quenched probesDroplet digital PCREwing sarcomaGenomic fusion sequencesLiquid biopsySerum marker

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Main Results:

  • Successfully identified and utilized genomic fusion sequences as liquid biopsy markers.
  • Demonstrated the potential for noninvasive monitoring in Ewing sarcoma patients.
  • Provided a viable alternative for tumors lacking conventional serum markers.

Conclusions:

  • Genomic fusion sequences are effective liquid biopsy markers for Ewing sarcoma.
  • This method enables noninvasive therapy monitoring, especially when serum markers are unavailable.
  • Advances in liquid biopsy offer new avenues for pediatric cancer management.