Jove
Visualize
Contact Us

Related Concept Videos

Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

1.7K
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
1.7K
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

906
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
906
Adrenergic Agonists: Indirect-Acting Agents01:25

Adrenergic Agonists: Indirect-Acting Agents

2.4K
Indirect-acting adrenergic agonists potentiate the effects of endogenous catecholamines through different mechanisms without directly binding to adrenoceptors.
One mechanism involves depleting stored catecholamines by displacing them from synaptic vesicles. These agents, known as "displacers," are transported into vesicles at the expense of noradrenaline. Examples include amphetamine and tyramine, which lack a catechol moiety, resulting in prolonged action, improved oral...
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiac adverse events following post-operative radiation therapy in patients with thymic malignancies.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2026
Same author

A mixed methods systematic review on the impact of COVID-19 on healthcare workers' knowledge, attitudes and practices of infection prevention and control in the UK.

The Journal of hospital infection·2026
Same author

Development of a history-taking form for mesothelioma patients at risk of exposure to asbestos.

Public health action·2025
Same author

Machine learning to predict complications after salvage surgery in head and neck cancers.

International journal of oral and maxillofacial surgery·2025
Same author

Anatomical and physiological diagnostic discrepancies in fetuses with single-ventricle congenital heart disease in a contemporary cohort.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2024
Same author

Evaluating the effect of child home safety training upon three family support practitioner groups: a mixed-methods study.

Perspectives in public health·2023
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 25, 2025

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.0K

Kavain Interference with Amphetamine Immunoassay.

H Madhavaram1, T Patel1, C Kyle1

  • 1LabPlus, Auckland City Hospital, Auckland, New Zealand.

Journal of Analytical Toxicology
|December 16, 2020
PubMed
Summary
This summary is machine-generated.

Kavain, found in the kava drink, can cause false positive amphetamine test results in urine immunoassays. Gas chromatography-mass spectrometry confirmed the presence of kavain, not amphetamines, in patient samples.

More Related Videos

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

3.6K
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

15.0K

Related Experiment Videos

Last Updated: Nov 25, 2025

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.0K
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

3.6K
A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

15.0K

Area of Science:

  • Forensic Toxicology
  • Analytical Chemistry

Background:

  • Kava (Piper methysticum) consumption is prevalent in Oceania and Australasia.
  • Kavain is a kava-lactone found in kava, known for ceremonial and recreational use.
  • Unexpected false positive urine immunoassay results for amphetamine-type substances were observed in three patients.

Purpose of the Study:

  • To investigate the cause of false positive amphetamine immunoassay results.
  • To identify the substance responsible for cross-reactivity with amphetamine assays.
  • To determine if kavain, a kava component, interferes with drug testing.

Main Methods:

  • Urine samples were analyzed using Beckman Coulter AU480 immunoassay and gas chromatography-mass spectrometry (GC-MS).
  • Kava powders and authentic kavain standard were tested for cross-reactivity.
  • Kavain standard was analyzed by GC-MS to compare mass fragmentation and retention time.

Main Results:

  • Patient urine samples tested positive for amphetamines by immunoassay but were negative for amphetamine-type substances by GC-MS, containing only kavain.
  • Kava powders and kavain standard demonstrated cross-reactivity with the amphetamine immunoassay, yielding false positive results.
  • GC-MS confirmed the absence of amphetamine-type compounds in kava powders and kavain standard.

Conclusions:

  • Kavain is identified as the cause of false positive amphetamine immunoassay results.
  • This is the first reported instance of kavain causing false positive amphetamine measurements.
  • Clinical laboratories should be aware of potential interference from kava consumption in drug testing.