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Dynamically tunable light responsive silk-elastin-like proteins.

Om Prakash Narayan1, Xuan Mu1, Onur Hasturk1

  • 1Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.

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Summary
This summary is machine-generated.

Researchers developed new photoresponsive hydrogels using genetically engineered Silk-Elastin-Like Proteins (SELP) and a light-sensitive protein domain. These materials change properties under visible light, showing potential for biomedical applications like drug delivery and tissue regeneration.

Keywords:
BioengineeringBiomaterialsElastinSilkSilk-elastin-like proteinsStimuli-responsive

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Protein Engineering

Background:

  • Dynamically tunable biomaterials are crucial for advanced biomedical applications.
  • Stimuli-responsive protein hydrogels offer genetic tailorability and control over structure-function relationships.
  • Silk-Elastin-Like Proteins (SELP) are promising candidates for engineered biomaterials.

Purpose of the Study:

  • To synthesize genetically engineered Silk-Elastin-Like Protein (SELP) photoresponsive hydrogels.
  • To investigate the photoresponsive behavior of SELP-CarHC hydrogels.
  • To evaluate the biocompatibility and cell encapsulation capabilities of the developed hydrogels.

Main Methods:

  • Genetically engineered SELPs were polymerized with the CarHC photoreceptor domain.
  • SpyTag-SpyCatcher peptide-protein pairs were used for polymerization under physiological conditions.
  • Hydrogel mechanical properties (loss and storage moduli) were measured before and after light exposure.
  • Cytotoxicity and fibroblast encapsulation/release were assessed.

Main Results:

  • Successful synthesis of SELP-CarHC photoresponsive hydrogels.
  • Hydrogels exhibited a partial collapse of the network upon visible light exposure.
  • A decrease in both loss and storage moduli was observed after light stimulation.
  • The hydrogels demonstrated low cytotoxicity and facilitated encapsulation and release of L929 murine fibroblasts.

Conclusions:

  • Novel photoresponsive SELP-CarHC hydrogels were successfully designed and synthesized.
  • These materials offer dynamic tunability in response to visible light.
  • The developed hydrogels show promise for applications in drug delivery, cell delivery, and tissue regeneration.