Related Experiment Video

Updated: Nov 25, 2025

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

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Type I Interferon (IFN)-Regulated Activation of Canonical and Non-Canonical Signaling Pathways

Candice Mazewski1,2, Ricardo E Perez1,2, Eleanor N Fish3

  • 1Robert H. Lurie Comprehensive Cancer Center of Northwestern University, Chicago, IL, United States.

Frontiers in Immunology
|December 17, 2020
PubMed

For several decades there has been accumulating evidence implicating type I interferons (IFNs) as key elements of the immune response. Therapeutic approaches incorporating different recombinant type I IFN proteins have been successfully employed to treat a diverse group of diseases with significant and positive outcomes. The biological activities of type I IFNs are consequences of signaling events occurring in the cytoplasm and nucleus of cells. Biochemical events involving JAK/STAT proteins that control transcriptional activation of IFN-stimulated genes (ISGs) were the first to be identified and are referred to as "canonical" signaling. Subsequent identification of JAK/STAT-independent signaling pathways, critical for ISG transcription and/or mRNA translation, are denoted as "non-canonical" or "non-classical" pathways. In this review, we summarize these signaling cascades and discuss recent developments in the field, specifically as they relate to the biological and clinical implications of engagement of both canonical and non-canonical pathways.

Keywords:
COVID-19MAP kinase signalingSARS-CoV-2interferonmRNA translationmammalian target of rapamycinsignal transducer and activator of transcriptionsignaling

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