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Updated: Nov 25, 2025
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Hydrophobic deep eutectic solvent-based ultrasonic-assisted liquid-liquid micro-extraction combined with HPLC-FLD for
Shaomin Ma1, Xiuyi Jin1, Huan Wei1
1College of Environment and Chemical Engineering, Dalian University , Dalian, China.
Abstract:
In this paper, novel high extraction efficiency hydrophobic eutectic solvents (DESs), n-octanoic acid as a hydrogen bond donor (HBD) and menthol as a hydrogen bond acceptor (HBA), were selected from five hydrophobic DESs to extract trace diphenylamine (DPA) in fruits apple, pear and orange under ultrasonic-assisted liquid-liquid micro-extraction (UA-LLME) technology before high-performance liquid chromatography (HPLC) analysis. Working parameters such as the DESs type, molar ratio, extractant volume, and ultrasonic time of the LLME hydrophobic DESs technology were optimised. Average recoveries between 96% and 108% were obtained on actual samples. This method gave lower detection limit (LOD) than other existing methods due to combining the high-efficiency extraction of hydrophobic DES and high sensitivity of fluorescence detector. This method was sensitive and eco-friendly, and can be used for the determination of trace components in fruits.
