A highly sensitive flow injection amperometric glucose biosensor using a gold nanoparticles/polytyramine/Prussian...

Area of Science:

• Electrochemistry
• Biosensors
• Nanomaterials

Background:

• Development of sensitive and selective glucose biosensors is crucial for diabetes management.
• Enzyme-based biosensors offer high specificity for glucose detection.
• Nanomaterials can enhance the performance of biosensors through increased surface area and improved electron transfer.

Purpose of the Study:

• To fabricate a novel amperometric glucose biosensor.
• To investigate the performance of the biosensor for glucose detection.
• To evaluate the biosensor's stability, reproducibility, and selectivity.

Main Methods:

• Fabrication of a glucose biosensor by immobilizing glucose oxidase (GOx) on gold nanoparticles (AuNPs) supported by a polytyramine (Pty) layer.
• Coating the assembly onto a Prussian blue (PB)-modified screen-printed carbon electrode (SPCE).
• Amperometric detection at -0.10 V in a flow injection analysis system.

Main Results:

• The biosensor exhibited linearity from 1.0 μM to 1.0 mM with a limit of detection of 1.0 μM.
• A low Michaelis constant (0.21 mM) indicated high GOx affinity for glucose.
• Excellent operational stability (374 injections), long-term stability (3 weeks), good reproducibility (1.9%-4.3% RSD), and high selectivity were observed.

Conclusions:

• The developed GOx/AuNPs/Pty/PB/SPCE biosensor is a promising tool for accurate and reliable glucose monitoring.
• The biosensor demonstrated satisfactory performance in analyzing glucose in real blood plasma samples.
• The fabrication approach offers a viable strategy for developing advanced enzyme-based biosensors.