Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.9K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.9K
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

959
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
959

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemical basis of fluorogenic substrate probe design: revisiting the past to discover the new in biochemistry.

RSC chemical biology·2026
Same author

The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.

JACS Au·2026
Same author

Solid-Phase Synthesis of ProTide Fluorogenic Probes Enables Systematic Profiling of Carboxypeptidase Activity.

Journal of the American Chemical Society·2026
Same author

Diversification of the 10th core atom of 9-cyanopyronins expands the resonance Raman vibrational palette.

Communications chemistry·2026
Same author

Correction to "Low-Background Cancer Imaging with a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety".

Journal of the American Chemical Society·2026
Same author

Multicenter ex vivo evaluation of an α-mannosidase-targeted fluorescent probe for intraoperative margin assessment in breast cancer.

Scientific reports·2026

Related Experiment Video

Updated: Nov 25, 2025

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.5K

Recent Progress in Small Spirocyclic, Xanthene-Based Fluorescent Probes.

Sascha G Keller1, Mako Kamiya1, Yasuteru Urano1,2,3

  • 1Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Molecules (Basel, Switzerland)
|December 19, 2020
PubMed
Summary

Small molecular, spirocyclic xanthene-based fluorescent probes show expanding applications. This review details their use in enzyme interactions, cellular labeling, small molecule detection, and advanced imaging techniques.

Keywords:
fluoresceinfluorescencerhodaminerhodolspirocyclicxanthene-based

More Related Videos

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

14.1K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.4K

Related Experiment Videos

Last Updated: Nov 25, 2025

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.5K
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

14.1K
Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
07:44

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

11.4K

Area of Science:

  • Chemical Biology
  • Molecular Imaging
  • Organic Chemistry

Background:

  • Fluorescent probes are crucial tools in various scientific disciplines.
  • Small molecular, spirocyclic xanthene-based structures are gaining attention for their unique properties.

Purpose of the Study:

  • To review recent advancements in small molecular, spirocyclic xanthene-based fluorescent probes.
  • To highlight their diverse applications and structure-behavior relationships.

Main Methods:

  • Literature review of recent progress in xanthene-based fluorescent probes.
  • Analysis of probe applications in biological systems and diagnostics.
  • Summary of synthetic strategies and computational approaches.

Main Results:

  • Xanthene-based probes demonstrate utility in enzyme interactions, organelle/protein targeting, and small molecule detection.
  • Applications extend to therapeutics, diagnostics, and super-resolution microscopy.
  • Advances in synthesis and structure-behavior understanding are presented.

Conclusions:

  • Spirocyclic xanthene-based fluorescent probes are versatile tools with a growing range of applications.
  • Continued research in synthesis and computational methods will further enhance their utility.