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Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...

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A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut.

Svetlana Ulyanchenko¹, Jordi Guiu^2,3,4

¹Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|December 19, 2020

Summary

Lineage tracing, using inducible recombinases and fluorescent reporters, identifies cell fates and stem cells in vivo. This method quantitatively analyzes cell populations for insights into tissue development and disease.

Keywords:

C-section Fetal intestine Intestinal stem cells Intestine Lineage tracing

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A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

Area of Science:

Developmental Biology
Stem Cell Biology
Cell Biology

Background:

Lineage-tracing experiments are crucial for understanding cell progeny and fate.
Inducible recombinases and fluorescent reporters are key tools for defining cellular hierarchies and identifying stem cells in vivo.
Refined lineage tracing enables quantitative analysis of clonal dynamics.

Purpose of the Study:

To describe a protocol for lineage tracing of fetal intestinal epithelium to the adult intestine.
To demonstrate the broad applicability of lineage tracing for assessing fetal cell fate during morphogenesis.
To provide a comprehensive in vivo analysis of cellular behavior.

Main Methods:

Utilizing inducible recombinases and fluorescent reporters for cell labeling.
Single-cell labeling and quantitative analysis of clonal dynamics.
Applying lineage tracing to fetal intestinal epithelium for tracking cell fate to adulthood.

Main Results:

Established cellular hierarchies in tissues.
Quantified the frequency of cell fate decisions on a population basis.
Enabled comparison of different stem cell populations and replenishment mechanisms.

Conclusions:

Lineage tracing provides a comprehensive analysis of cellular behavior in vivo.
This approach is widely applicable for quantitatively assessing fetal cell fate during morphogenesis.
The described protocol facilitates detailed study of stem cell populations and tissue development.