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(Re) Defining the High-Power Field for Digital Pathology.

David Kim1, Liron Pantanowitz2, Peter Schüffler3,4

  • 1Department of Pathology and Laboratory Medicine, New York-Presbyterian Hospital, Weill Cornell Medical College, New York, NY, USA.

Journal of Pathology Informatics
|December 21, 2020
PubMed
Summary
This summary is machine-generated.

Digital pathology high-power field (HPF) area varies with monitor size and resolution, not scanner type. This impacts diagnostic accuracy, necessitating new digital metrics for reliable evaluation.

Keywords:
Digital pathologygradehigh-power fieldmitosiswhole slide imaging

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Area of Science:

  • Digital pathology
  • Histopathology
  • Image analysis

Background:

  • Microscope high-power field (HPF) is crucial for histopathology diagnostics like mitotic figure quantification and tumor grading.
  • Traditional light microscopy evaluates HPFs at ×400 magnification across 10 fields of view.
  • Digital pathology introduces new variables affecting HPF evaluation.

Purpose of the Study:

  • To determine parameters influencing high-power field (HPF) evaluation in whole slide images (WSIs).

Main Methods:

  • Compared glass slides scanned on various devices to digital slides viewed on different WSI viewer software and an in-house viewer.
  • Reviewed WSIs at "×40" equivalent HPF on monitors with varying sizes, resolutions, and aspect ratios.
  • Calculated and compared digital and microscopic HPF areas.

Main Results:

  • Significant variation in HPF area was observed across different monitor sizes and display resolutions.
  • Minor differences in HPF area were noted between WSI viewers.
  • No significant differences were found based on the scanner or WSI scanning resolution.

Conclusions:

  • Microscopic HPF at ×400 magnification is not equivalent to "×40" digital HPF areas.
  • Digital HPF quantification is influenced by monitor size, display resolution, and WSI viewers.
  • Further clinical validation and development of new digital metrics are needed for accurate HPF evaluation.