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Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
A novel electrochemical biosensor for ultrasensitive Hg2+ detection via a triple signal amplification strategy
Wang He1, Bin Qiao2, Fengzhen Li1
1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China. wangxiaohong@hainu.edu.cn.
Abstract:
We developed a novel electrochemical biosensor for ultrasensitive Hg2+ detection via a triple signal amplification strategy of a DNA dual cycle, organic-inorganic hybrid nanoflowers (Cu3(PO4)2 HNFs) and gold nanoparticle (AuNP) probe. The DNA dual cycle was triggered by exonuclease III (Exo III) in the presence of Hg2+, and Cu3(PO4)2 HNFs were synthesized as an AuNP probe carrier. The electrochemical biosensor displayed high stability, high sensitivity and excellent specificity, which was improved by up to seven orders of magnitude compared to the World Health Organization (WHO) allowed Hg2+ levels in drinking water. This signal amplification strategy could be easily modified and extended to detect other hazardous heavy metals and nucleic acids.
