Near-infrared hyperspectral imaging for detection and visualization of offal adulteration in ground pork

Hyperspectral imaging (HSI) technique was investigated to explore a feasible protocol for detecting the potential offal (lung) adulteration in ground pork. Tested samples (176 adulterated and 2 controls) were first prepared with adulterant of ground lung in range of 0%-100% (w/w) at 10% intervals. After hyperspectral images were acquired and calibrated in reflectance mode (400-1000 nm), full spectra were extracted from identified regions of interests (ROIs) and then transformed into absorbance and Kubelka-Munck spectral units, respectively. Partial least squares regression (PLSR) models based on full spectra showed that raw reflectance spectra with no preprocessings performed best with coefficient of determination (R_p²) of 0.98, root mean square error (RMSEP) of 4.25%, and ratio performance deviation (RPD) of 7.53 in prediction set. To reduce the high dimensionality of spectra, data was further explored using principal component loadings, two-dimensional correlation spectroscopy (2D-COS), and regression coefficients (RC), respectively. The optimal performance of established simplified PLSR model were acquired using eleven featured wavelengths selected by PC loadings with R_p² of 0.98, RMSEP of 4.47% and RPD of 7.16. Finally, the limit of detection (LOD) was calculated to be a satisfactory 7.58%, and readily discernible visualization procedure using preferred simplified PLSR model yielded satisfactory spatial distribution of adulteration situation. Control samples with known distribution were also visualized to successfully prove the validity. Consequently, this research offers an alternative assay for visually and rapidly detecting offal of lung adulteration in ground pork.