Generation of a FOXH1 homozygous knockout human embryonic stem cell line by CRISPR/Cas9 system

Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous knockout human embryonic stem cell (hESC), WAe009-A-42 by CRISPR/Cas9 mediated gene targeting. The WAe009-A-42 retained a typical undifferentiated morphology and normal karyotype, pluripotenty, and trilineage differentiation potential in vivo and vitro.