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Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

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Related Experiment Video

Updated: Nov 24, 2025

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

GRF-GIF Chimeras Boost Plant Regeneration.

Guangbin Luo¹, Michael Palmgren¹

¹NovoCrops Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Denmark.

Trends in Plant Science

|December 22, 2020

Summary

Plant regeneration is improved by GROWTH-REGULATING FACTORs (GRFs) and GRF-INTERACTING FACTOR (GIF) chimeras. This breakthrough enhances plant transformation and gene editing efficiency across many species.

Area of Science:

Plant Biotechnology
Molecular Biology
Genetics

Background:

Plant regeneration from tissue culture is crucial for plant biotechnology.
Low regeneration efficiency is a significant bottleneck in plant transformation and genetic engineering.
GROWTH-REGULATING FACTORs (GRFs) are transcription factors involved in plant growth and development.

Purpose of the Study:

To investigate the role of GROWTH-REGULATING FACTORs (GRFs) in enhancing plant regeneration.
To evaluate the efficacy of GRFs alone and in combination with GRF-INTERACTING FACTORs (GIFs) for improving tissue culture regeneration.
To assess the potential of GRF-GIF chimeras as a tool for plant transformation and gene editing.

Main Methods:

Two independent studies were conducted to assess regeneration efficiency.

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Experiments involved the expression of GRFs and GRF-GIF chimeras in plant tissue cultures.

Regeneration rates were measured across a broad range of plant species.

Main Results:

GRFs significantly boosted regeneration efficiency in plant tissue cultures.
GRF-GIF chimeras demonstrated a dramatic improvement in regeneration across diverse species.
The findings indicate a broad applicability of GRF-GIF technology.

Conclusions:

GRFs, particularly in GRF-GIF chimeras, represent a powerful tool for enhancing plant regeneration.
This technology has the potential to revolutionize plant transformation and gene editing.
The improved regeneration efficiency could accelerate crop improvement and research.