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A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
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A 3D human adipose tissue model within a microfluidic device.

Feipeng Yang1, Alanis Carmona, Katerina Stojkova

  • 1Illinois Institute of Technology, Department of Biomedical Engineering, Chicago, 60616, USA.

Lab on a Chip
|December 22, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a 3D human adipose microtissue in a microfluidic system. This engineered tissue mimics in vivo conditions, enabling better study of adipocyte function and drug screening for metabolic diseases.

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Microfluidics

Background:

  • Traditional 2D cell cultures do not accurately mimic the 3D in vivo adipose environment.
  • Existing 3D culture models often struggle with long-term viability due to mass transport limitations.
  • Microfluidic systems offer potential for improved adipose tissue modeling, but have focused on 2D cultures.

Purpose of the Study:

  • To engineer a functional 3D human adipose microtissue within a microfluidic system.
  • To investigate the effects of shear stress on adipogenesis and secretion in the engineered tissue.
  • To establish a platform for in vitro drug testing related to adipose tissue diseases.

Main Methods:

  • Human adipose-derived stem cells (ADSCs) were differentiated into adipocytes within a microfluidic device.
  • The microfluidic system facilitated interstitial flow to support the 3D microtissue.
  • Shear stress levels were modulated to assess their impact on the engineered adipose tissue.

Main Results:

  • ADSCs successfully differentiated into a dense, lipid-loaded 3D adipose microtissue expressing key genetic markers.
  • Engineered adipose tissue exhibited decreased adiponectin and increased free fatty acid secretion with rising shear stress.
  • Adipogenesis markers were downregulated as shear stress increased.

Conclusions:

  • The microfluidic system successfully supports on-chip differentiation and development of functional 3D human adipose microtissue.
  • Interstitial flow within the microfluidic system is crucial for tissue development and function.
  • This engineered 3D adipose microtissue platform holds promise for in vitro drug screening for adipose tissue-related disorders.