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Updated: Nov 24, 2025

Overexpressing and Purifying a Toxic Nuclease from Escherichia coli
Published on: August 29, 2025
Overexpression of SARS-CoV-2 protein ORF6 dislocates RAE1 and NUP98 from the nuclear pore complex
Koki Kato1, Dini Kurnia Ikliptikawati1, Akiko Kobayashi2
1School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan.
Abstract:
The novel human betacoronavirus SARS-CoV-2 has caused an unprecedented pandemic in the 21st century. Several studies have revealed interactions between SARS-CoV-2 viral proteins and host nucleoporins, yet their functions are largely unknown. Here, we demonstrate that the open-reading frame 6 (ORF6) of SARS-CoV-2 can directly manipulate localization and functions of nucleoporins. We found that ORF6 protein disrupted nuclear rim staining of nucleoporins RAE1 and NUP98. Consequently, this disruption caused aberrant nucleocytoplasmic trafficking and led to nuclear accumulation of mRNA transporters such as hnRNPA1. Ultimately, host cell nucleus size was reduced and cell growth was halted.
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