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Sperm Collection of Differential Quality Using Density Gradient Centrifugation
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Bio-Functional Sperm Parameters: Does Age Matter?

Rosita A Condorelli1, Sandro La Vignera1, Federica Barbagallo1

  • 1Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.

Frontiers in Endocrinology
|December 28, 2020
PubMed
Summary
This summary is machine-generated.

Male aging negatively impacts sperm quality, increasing DNA fragmentation. Mitochondrial damage and poor chromatin compactness contribute to apoptosis, elevating sperm DNA fragmentation rates in men.

Keywords:
alive spermatozoamale agesperm DNA fragmentationsperm apoptosissperm lipid peroxidationsperm mitochondrial membrane potentialspermatozoa

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Area of Science:

  • Reproductive biology
  • Spermatozoa
  • Male infertility

Background:

  • Idiopathic male infertility can be explained by biofunctional sperm parameters.
  • Sperm DNA fragmentation (fDNA) is a key parameter.
  • Mitochondrial membrane potential (MMP), apoptosis, lipid peroxidation (LP), and mitochondrial superoxide are important indicators.

Purpose of the Study:

  • To investigate the correlation between male age and biofunctional sperm parameters.
  • To assess the impact of these parameters on sperm DNA fragmentation (fDNA).

Main Methods:

  • Flow cytometry was used to evaluate MMP, chromatin compactness, apoptosis/vitality, fDNA, LP, and mitochondrial superoxide.
  • 874 men were included in the cohort.

Main Results:

  • Age negatively correlated with live spermatozoa (r = -0.75, p < 0.05).
  • Low MMP and abnormal chromatin compactness were linked to reduced live spermatozoa and increased apoptosis.
  • Increased fDNA correlated with PS externalization and decreased live spermatozoa.

Conclusions:

  • Male aging, mitochondrial damage, and altered chromatin compactness may trigger apoptosis.
  • This cascade can lead to increased sperm DNA fragmentation (fDNA).