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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Nov 24, 2025

Autofluorescence Imaging to Evaluate Cellular Metabolism

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging.

Yutong Zhang¹, Yulin Du¹, Yuting Zhuo^1,2

¹Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, China.

Frontiers in Chemistry

|December 28, 2020

Summary

This summary is machine-generated.

Functional nucleic acids (FNAs) are emerging as powerful tools for live-cell imaging. This review highlights their applications in visualizing biological processes within living cells, advancing disease theranostics.

Keywords:

DNAzyme aptamer fluorescence probe functional nucleic acid live-cell imaging

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Last Updated: Nov 24, 2025

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Area of Science:

Cell biology
Molecular biology
Biotechnology

Background:

Cells are fundamental units of life, crucial for understanding biological processes like metabolism and stress.
Live-cell imaging is vital for studying molecular functions and disease mechanisms.
Developing advanced molecular probes for imaging has been a significant challenge.

Purpose of the Study:

To review the applications of functional nucleic acids (FNAs) in live-cell fluorescence imaging.
To highlight the potential of FNAs as molecular tools for cellular research and diagnostics.

Main Methods:

Focuses on a mini-review of existing literature on FNA applications in live-cell imaging.
Discusses the use of aptamers and DNAzymes as examples of FNAs.

Main Results:

Functional nucleic acids (FNAs) show great promise as molecular probes for live-cell imaging.
These nucleic acid-based tools can be engineered for specific molecular targeting and function.
Their application facilitates the study of spatiotemporal distribution and activity of molecules in living cells.

Conclusions:

FNAs offer a novel and effective approach to overcome limitations in current molecular probe development.
Their use in live-cell fluorescence imaging is crucial for advancing our understanding of life mechanisms.
FNAs hold significant potential for disease pathogenesis elucidation and theranostics.