Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A pseudoknotted RNA oligonucleotide.

J D Puglisi1, J R Wyatt, I Tinoco

  • 1Department of Chemistry, University of California, Berkeley 94720.

Nature
|January 21, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biophysical analysis of nucleic acids.

Current protocols in nucleic acid chemistry·2008
Same author

Force unfolding kinetics of RNA using optical tweezers. II. Modeling experiments.

Biophysical journal·2007
Same author

Verification of the Crooks fluctuation theorem and recovery of RNA folding free energies.

Nature·2005
Same author

The effect of force on thermodynamics and kinetics: unfolding single RNA molecules.

Biochemical Society transactions·2004
Same author

[A 65-year-old woman with mediastinal enlargement].

Revista clinica espanola·2004
Same author

Efficacy of directly observed treatment of HIV infection: experience in AIDS welfare homes.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2004
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
See all related articles

RNA pseudoknots are a novel tertiary structure crucial for RNA folding. This study provides evidence that short RNA oligonucleotides can form stable pseudoknotted structures, impacting RNA function.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • RNA tertiary structure enables diverse functions, including enzymatic and regulatory roles.
  • Predicting RNA secondary structure is established, but tertiary structure and thermodynamics remain less understood.
  • Pseudoknots, a novel RNA folding pattern, have been proposed in viral RNAs.

Purpose of the Study:

  • To investigate the formation and stability of RNA pseudoknotted structures.
  • To present evidence for pseudoknot formation in a short RNA oligonucleotide.
  • To compare the properties of pseudoknotted structures with hairpin structures.

Main Methods:

  • Utilized single-strand specific (S1) and double-strand specific (V1) nuclease digestion.
  • Analyzed thermodynamic properties of RNA oligonucleotides.

Related Experiment Videos

  • Compared digestion patterns and thermodynamics of pseudoknotted and hairpin structures.

    • Main Results:

    • Demonstrated that a 19-nucleotide RNA oligonucleotide adopts a stable pseudoknotted structure.
    • Showed that specific sequences can indeed form pseudoknots.
    • Identified pseudoknots as a significant type of local tertiary structure.

    Conclusions:

    • Pseudoknots represent a stable and important local tertiary structure in RNA.
    • These structures must be considered when analyzing the folding of complex RNA molecules.
    • The findings contribute to understanding RNA structure-function relationships.