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Updated: Nov 24, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Next-generation sequencing yields the complete chloroplast genome of Pleione forrestii (Orchidaceae)
Sha-Sha Wu1,2, Xiao-Qian Wu1,2, Na Dong1,2
1Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.
Abstract:
Pleione forrestii is an endangered terrestrial orchid with significant ornamental values. Here, we report the first complete chloroplast genome of P. forrestii. The circular genome was 158,759 bp in length and consisted of a pair of inverted repeats (IR 26,429 bp), which were separated by a large single-copy region (LSC 87,236 bp) and a small single copy-region (SSC 18,665 bp). It contained 135 genes (115 unique), including 87 protein-coding genes, 38 tRNAs, and 8 rRNAs. The maximum likelihood phylogenetic analysis indicated that P. forrestii was sister to P. bulbocodioides and P. formosana.
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