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Updated: Nov 24, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome of an endangered plant-Nomocharis aperta
Xueda Chen1,2, Xiuhai Zhang2, Yixin Liu2
1Resources and Environment College, Tibet Agriculture and Animal Husbandry University, Nyingchi, China.
Abstract:
Nomocharis aperta is an endangered and endemic species with high ornamental value in China. In this study, we reported a complete chloroplast genome of N. aperta, which was de novo assembled using the next-generation sequencing data. The complete chloroplast genome is 152,845 in length, including a large single copy region of 70,506 bp and a small single copy region of 17,468 bp and two inverted repeat regions of 26,520 bp. A total of 130 functional genes were encoded, consisting of 84 protein-coding genes, 36 transfer RNA genes, and 8 ribosomal RNA genes. The overall AT content of the chloroplast genome is 63.00%. In addition, phylogenetic analysis with the reported chloroplast genomes showed that N. aperta is nested within Lilium and close to L. henricii, L. bakerianum and L. taliense. It indicates that the study on the relationship between Nomocharis and Lilium needs more Nomocharis and Lilium complete chloroplast genome, especially some key species like N. aperta.
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