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Updated: Nov 24, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Complete chloroplast genome of Disporum sessile
Inkyu Park1, Goya Choi1, Sungyu Yang1
1Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Naju, Republic of Korea.
Abstract:
Disporum sessile roots are used as a medicinal herb. To ensure the accurate identification of D. sessile, we sequenced its chloroplast genome using the Illumina MiSeq platform. Results showed that the chloroplast genome of D. sessile is 159,102 bp in size, with a large single-copy (LSC) region (85,456 bp), a small single-copy (SSC) region (17,108 bp), and two inverted repeat (IR) regions (IRa and IRb; 28,269 bp each). Additionally, the chloroplast genome contains 112 unique genes, including 78 protein-coding, four ribosomal RNA, and 30 transfer RNA genes. Phylogenetic analysis revealed that D. sessile is most closely related to Colchicum autumnale.
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