Related Experiment Video
Updated: Nov 24, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
The complete chloroplast genome of Protea kilimandscharica Engl. (Proteaceae)
John M Nzei1,2,3, Josphat K Saina1,2,3, Virginia M Mwanzia1,2,3
1Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, Hubei, China.
Abstract:
Protea kilimandscharica is endemic to the heath zone of Mt Kenya, restricted to the rocky slopes of the mountain. The complete chloroplast genome of P. kilimandscharica was determined by next-generation sequencing technology, with a total length of 158,657 bp. The cp genome encodes 115 unique genes, with four rRNA genes, 81 protein-coding genes (PCGs), and 30 tRNA genes. A 3.1 kb inversion was noted in the LSC. Phylogenetic analysis, based on 75 common protein-coding genes revealed P. kilimandscharica as sister to Macadamia integrifolia and Macadamia ternifolia.
Related Concept Videos
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Export of Mitochondrial and Chloroplast Genes
Protein Transport to the Inner Chloroplast Membrane
Anatomy of Chloroplasts
The Anatomy of Chloroplasts
Structure of...
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.

