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A near-infrared fluorescent probe based on a hemicyanine dye with an oxazolidine switch for mitochondrial pH
Wafa Mazi1, Yunnan Yan, Yibin Zhang
1Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA. yibinz@mtu.edu rluck@mtu.edu mtajiri@mtu.edu hyliu@mtu.edu.
Abstract:
A near-infrared fluorescent probe (AH+) has been prepared by incorporating an oxazolidine switch into a near-infrared hemicyanine dye. The probe shows fast and sensitive responses to pH from an oxazolidine switch to the hemicyanine dye upon pH decreases from 10.0 to 5.0. The probe shows good photostability, low cytotoxicity, and reversible fluorescence responses to pH changes with a pKa value of 7.6. It has been successfully used to determine pH changes in mitochondria.
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