Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Nov 24, 2025

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Published on: June 17, 2021

MicroED Sample Preparation and Data Collection For Protein Crystals.

Guanhong Bu^1,2, Brent L Nannenga^3,4

¹Chemical Engineering, School for Engineering of Matter, Transport and Energy, Arizona State University, Tempe, AZ, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 28, 2020

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-throughput automated molecular replacement for small-molecule MicroED data.

IUCrJ·2026

Same author

Conformational Trajectory of the Molecular Chameleon Grazoprevir From Formulation to Target-Bound.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025

Same author

Hydroxamate Linkage Transformation in Porphyrin Functionalized Metal-Organic Frameworks.

Inorganic chemistry·2025

Same author

Molecular chameleons adaptability in target binding.

Structural dynamics (Melville, N.Y.)·2025

Same author

The 2024 challenges in structural biology summit.

Structural dynamics (Melville, N.Y.)·2025

Same author

Mechanochromic luminescence in copper nanoclusters: resolving structural transitions through microcrystal electron diffraction.

Chemical communications (Cambridge, England)·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Microcrystal Electron Diffraction (MicroED) allows determining the structure of tiny protein crystals. This study provides essential protocols for sample preparation and data collection using MicroED on standard cryo-TEM instruments.

Area of Science:

Crystallography
Structural Biology
Electron Microscopy

Background:

Conventional diffraction techniques are limited by crystal size.
Microcrystal Electron Diffraction (MicroED) overcomes these limitations.
MicroED is applicable to protein and small organic molecule structure determination.

Purpose of the Study:

To present detailed protocols for MicroED.
To facilitate structure determination of protein microcrystals.
To enable researchers to utilize standard cryo-TEM instruments for MicroED.

Main Methods:

Sample preparation techniques for protein microcrystals.
Data collection strategies for electron diffraction.
Utilizing high-speed detectors on cryo-transmission electron microscopy (cryo-TEM) instruments.

Keywords:

MicroED Microcrystal electron diffraction Protein crystallography Sample preparation Structural biology

More Related Videos

$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

$Microcrystal Electron Diffraction of Small Molecules$

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

Related Experiment Videos

Last Updated: Nov 24, 2025

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Published on: June 17, 2021

$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

$Microcrystal Electron Diffraction of Small Molecules$

Microcrystal Electron Diffraction of Small Molecules

Published on: March 15, 2021

Main Results:

Established robust protocols for MicroED sample preparation.
Defined optimal data collection parameters for protein microcrystals.
Demonstrated the feasibility of protein structure determination using standard cryo-TEM.

Conclusions:

MicroED is a powerful technique for small crystal structure analysis.
The presented protocols simplify MicroED implementation.
This work advances the application of MicroED in structural biology.