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Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
Published on: August 25, 2011
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Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos
Ahilya N Sawh1, Susan E Mango1
1Biozentrum, University of Basel, 4056 Basel-Stadt, Switzerland.
STAR Protocols
|December 30, 2020
Summary
This study presents a high-throughput DNA fluorescence in situ hybridization method to map chromosome structures in Caenorhabditis elegans embryos. The technique reveals in vivo chromosome organization and its variability at single-cell resolution.
Area of Science:
- Genetics
- Molecular Biology
- Developmental Biology
Background:
- Understanding chromosome conformation is crucial for gene regulation and cellular function.
- Existing methods for analyzing chromosome structure in vivo can be limited in throughput and resolution.
- Caenorhabditis elegans is a powerful model organism for studying fundamental biological processes.
Purpose of the Study:
- To develop a high-throughput and multiplexed DNA fluorescence in situ hybridization (FISH) method.
- To enable the tracing of chromosome conformation in Caenorhabditis elegans embryos.
- To generate single-cell and single-chromosome localization data for assessing in vivo structures.
Main Methods:
- High-throughput and multiplexed DNA fluorescence in situ hybridization (FISH).
- Application in Caenorhabditis elegans embryos.
- Generation of single-cell and single-chromosome localization data.
Main Results:
- The protocol successfully traces chromosome conformation in C. elegans embryos.
- It provides data on chromosome structure at single-cell resolution.
- The method allows assessment of the heterogeneity of chromosome structures in vivo.
Conclusions:
- This flexible FISH strategy can interrogate chromosome structure at various genomic scales.
- The protocol is valuable for studying chromosome organization and dynamics.
- It offers a robust approach for analyzing chromosome conformation in vivo.
