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Related Experiment Video

Updated: Nov 23, 2025

Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

39.4K

Functional Multiple-Spine Calcium Imaging from Brain Slices.

Tomoe Ishikawa1, Chiaki Kobayashi1, Naoya Takahashi1

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.

STAR Protocols
|December 30, 2020
PubMed
Summary

This study presents a novel protocol for visualizing synaptic inputs in neurons. It allows high-speed optical recording of calcium signals from hundreds of dendritic spines simultaneously.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biophysics

Background:

  • Excitatory inputs are primarily received at dendritic spines in postsynaptic neurons.
  • Understanding dendritic information processing requires detailed analysis of synaptic input dynamics along dendrites.

Purpose of the Study:

  • To develop and present a protocol for high-speed, wide-field optical recording of synaptic inputs in cultured neuronal networks.
  • To enable simultaneous detection of synaptic activity as calcium signals from numerous dendritic spines.

Main Methods:

  • Combines spinning-disk confocal microscopy with whole-cell patch-clamp recording.
  • Utilizes a calcium indicator loaded into neurons within ex vivo cultured networks.
  • Facilitates simultaneous optical recording from hundreds of spines across multiple dendritic branches.

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Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
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Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

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Horizontal Hippocampal Slices of the Mouse Brain
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Related Experiment Videos

Last Updated: Nov 23, 2025

Functional Calcium Imaging in Developing Cortical Networks
16:33

Functional Calcium Imaging in Developing Cortical Networks

Published on: October 22, 2011

39.4K
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

11.2K
Horizontal Hippocampal Slices of the Mouse Brain
08:59

Horizontal Hippocampal Slices of the Mouse Brain

Published on: September 22, 2020

19.9K

Main Results:

  • Successfully demonstrates a protocol for simultaneous, high-speed optical detection of synaptic inputs.
  • Enables visualization of synaptic activity as calcium transients in a large number of dendritic spines.
  • Provides a method to scrutinize spatiotemporal dynamics of synaptic inputs along dendrites.

Conclusions:

  • The presented protocol offers a powerful tool for investigating dendritic information processing.
  • Allows for detailed analysis of synaptic input integration at the single-spine level.
  • Facilitates advancements in understanding neural circuit function and computation.