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A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates
Andrey A Petropavlovskiy1, Michael G Tauro2, Patrick Lajoie3
1Department of Biology, The University of Western Ontario, London, ON N6A 3K7, Canada.
STAR Protocols
|December 30, 2020
Summary
This study introduces a simple, quantitative method for evaluating yeast cell growth and survival using spotting assays. The protocol precisely measures cell density in spots to detect subtle growth differences, aiding yeast research.
Area of Science:
- Microbiology
- Cell Biology
- Molecular Biology
Background:
- Yeast cell growth and survival are critical parameters in various biological studies.
- Accurate quantification of these parameters is essential for reproducible research.
- Existing methods may lack sensitivity or accessibility for certain applications.
Purpose of the Study:
- To present a detailed protocol for evaluating yeast cell growth and survival.
- To introduce a quantitative analysis of spotting assays for precise measurement.
- To provide an accessible method for both yeast experts and non-experts.
Main Methods:
- Quantitative analysis of yeast spotting assays.
- Measurement of cell density within defined spot sizes on assay images.
- Protocol designed for low-throughput applications and adaptability.
Main Results:
- The method reproducibly detects and quantifies subtle differences in yeast cell growth.
- Accurate assessment of cell density within spots allows for sensitive detection.
- The protocol is user-friendly and adaptable to specific experimental needs.
Conclusions:
- This protocol offers a robust and accessible approach for evaluating yeast cell growth and survival.
- Quantitative spotting assay analysis provides a sensitive tool for biological research.
- The method facilitates reproducible and precise measurements in yeast studies.
