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Updated: May 29, 2026

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
05:51

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Published on: December 19, 2011

Investigating substrate specificity in arogenate versus prephenate dehydratases.

Emily J Clayton1,2, Megan Smith-Uffen3, Travis W Tribble3

  • 1Department of Biology, University of Western Ontario, 1151 Richmond Street North, London, ON, N6A 5B7, Canada. emily.clayton@concordia.ca.

BMC Biology
|May 28, 2026
PubMed
Summary

Amino acid combinations in the regulatory ACT domain of Arabidopsis thaliana arogenate dehydratases (AtADTs) determine both arogenate dehydratase (ADT) and prephenate dehydratase (PDT) activity. This finding clarifies substrate specificity in phenylalanine biosynthesis.

Keywords:
Arabidopsis thalianaSaccharomyces cerevisiaeACAI assayACT domainArogenate dehydratasePrephenate dehydrataseScreening by complementationSequence-function relationship

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