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Updated: Nov 23, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
[Correlation between microRNA-34b/c single nucleotide polymorphism rs4938723 and male infertility]
Fan Wang1, Lei Wang1, Qiu-Yue Wu2
1School of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210023, China.
Objective:
To investigate the relationship between microRNA-34b/c single nucleotide polymorphism (SNP) rs4938723 and the risk of male infertility.
Methods:
This case-control study included 553 males aged 19-40 (29.42 ± 5.09) years with idiopathic infertility, 153 with azoospermia and 400 with oligoasthenospermia, and another 332 normal fertile men aged 19-40 (28.5 4 ± 4.63) years as controls. We collected the clinical data and peripheral blood samples from the subjects, genotyped microRNA-34b/c rs4938723 by Sequenom MassARRAY, and analyzed the relationship between the genotypes of microRNA-34b/c rs4938723 and the risk of male infertility using the logistic regression model.
Results:
Statistically significant differences were observed between the infertility patients and normal controls in sperm concentration ([18.71 ± 15.19] vs [79.91 ± 43.96] × 10⁶/ml, P < 0.01), the percentage of progressively motile sperm ([13.27 ± 24.52]% vs [42.82 ± 8.86]%, P < 0.01) and the level of follicle stimulating hormone ([16.09 ± 17.37] vs [12.20 ± 4.73] IU/L, P < 0.01). Compared with the TT genotype, the TC and CC genotypes showed no correlation with male infertility, nor did the genetic locus in the subgroup analysis.
Conclusions:
No correlation was found between microRNA-34b/c SNP rs4938723 and male infertility, which, however, needs to be further verified by larger-sized samples.
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