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Long-chain non-coding RNA LINC01554 promotes NGFR expression and inhibits cell proliferation, migration, and invasion
1Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Nantong University; the First People's Hospital of Nantong City, Nantong, China. newway82@ntu.edu.cn.
Objective:
The aim of this study was to analyze the role of LINC01554 in the pathogenesis of hepatocellular carcinoma (HCC) and explore the potential mechanism through which LINC01554 affects the migration and proliferation of HCC cells.
Patients And Methods:
LINC01554 expression in HCC tissues and its link to the prognosis of patients were analyzed by The Cancer Genome Atlas (TCGA) database. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was carried out to examine LINC01554 levels in 60 cases of HCC clinical tissues and HCC cell lines. Then, LINC01554 overexpression model was constructed using lentivirus in HCC cell lines. HCC proliferation and invasive ability were evaluated through Cell Counting Kit (CCK-8) and transwell tests, respectively. Furthermore, the potential action mechanism of LINC01554 was explored using bioinformatics analysis and in vitro cell experiments.
Results:
Analysis of the TCGA database revealed that LINC01554 was remarkably under-expressed in HCC tissues. Decreased expression of LINC01554 predicted a poor prognosis for patients. Besides, LINC01554 overexpression markedly blunted the proliferation and migratory capacities of HCC cells. LINC01554 competed with NGFR to bind to microRNA-3681-3p, thereby providing possible mechanisms by which LINC01554 could participate in the progression of HCC.
Conclusions:
This study shows for the first time that LINC01554 modulates NGFR expression by binding to microRNA-3681-3p, thereby participating in the progression of HCC.
Insights
This study reveals that low LINC01554 expression is linked to poor hepatocellular carcinoma (HCC) prognosis. LINC01554 suppresses HCC cell proliferation and migration by interacting with microRNA-3681-3p and NGFR.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Hepatocellular carcinoma (HCC) is a major global health concern.
- Understanding the molecular mechanisms driving HCC progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the role of LINC01554 in hepatocellular carcinoma (HCC) pathogenesis.
- To explore the mechanism by which LINC01554 influences HCC cell migration and proliferation.
Main Methods:
- Utilized The Cancer Genome Atlas (TCGA) database for expression analysis.
- Employed quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for LINC01554 level assessment.
- Conducted in vitro experiments including CCK-8, transwell assays, and bioinformatics analysis.
Main Results:
- LINC01554 was found to be significantly underexpressed in HCC tissues, correlating with poor patient prognosis.
- Overexpression of LINC01554 inhibited HCC cell proliferation and migration.
- LINC01554 was identified to compete with NGFR for binding to microRNA-3681-3p.
Conclusions:
- LINC01554 acts as a tumor suppressor in HCC.
- This study elucidates a novel mechanism where LINC01554 regulates NGFR expression via microRNA-3681-3p, impacting HCC progression.
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