Related Experiment Video

Updated: Nov 23, 2025

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
08:13

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Published on: December 27, 2016

19.2K

Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell

Alberto Montalbano1, Cesare Sala1, Chiara Abrardo1

  • 1Department of Experimental and Clinical Medicine, University of Florence, I‑50134 Florence, Italy.

Data in Brief
|January 1, 2021
PubMed

Insights

This study examined potassium channel activity in human lymphoma cells using specific drug blockers. Findings offer insights into potassium channel function in these cancer cells.

Area of Science:

  • Electrophysiology
  • Molecular Biology
  • Oncology

Background:

  • Potassium channels, specifically Kv1.3 and KCa3.1, are crucial for lymphoid cell function.
  • Dysregulation of these channels is implicated in various cancers, including lymphomas.

Purpose of the Study:

  • To investigate the effects of riluzole, Psora-4, and Tram-34 on Kv1.3 and KCa3.1 potassium currents in human lymphoma cell lines.
  • To characterize the functional roles of these potassium channels in lymphoma cellular models.

Main Methods:

  • Whole-cell patch-clamp technique in voltage clamp mode was employed.
  • Potassium currents were measured using voltage ramps from -120 mV to +40 mV.
  • Specific channel blockers Psora-4 (for Kv1.3) and Tram-34 (for KCa3.1) were used, with KCa3.1 activation by riluzole.

Main Results:

  • The study measured the conductance of Kv1.3 and KCa3.1 channels in the presence and absence of specific blockers.
  • Riluzole was used to activate KCa3.1 channels before Tram-34 application.
  • Data provides a baseline for understanding potassium channel activity in lymphoma.

Conclusions:

  • The findings provide essential data on the functional characteristics of Kv1.3 and KCa3.1 potassium channels in human lymphoma.
  • This research serves as a foundation for further studies on potassium conductance in lymphoma tumor models.
Keywords:
Human lymphomasKCa3.1Kv1.3Patch-clampPotassium channels

More Related Videos

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

15.4K
Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique08:11

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Published on: November 11, 2022

3.2K

Related Concept Videos

Antiarrhythmic Drugs: Class III Agents as Potassium Channel Blockers01:12

Antiarrhythmic Drugs: Class III Agents as Potassium Channel Blockers

Class III antiarrhythmic drugs are a group of medications that can prolong action potentials in the heart. They achieve this by blocking potassium channels or enhancing inward currents from sodium channels. However, these drugs have a unique property of "reverse use-dependence," which is most pronounced at slower heart rates and can lead to torsades de pointes—a specific type of arrhythmia. However, it is essential to note that excessive QT interval prolongation—a measure of...
1.6K
Antiepileptic Drugs: Potassium Channel Activators01:20

Antiepileptic Drugs: Potassium Channel Activators

Ezocgabine or retigabine, an antiepileptic drug of remarkable efficacy, has revolutionized the management of seizures. It is a potassium channel activator, explicitly targeting the family of Q subtype potassium channels. It enhances the transmembrane potassium currents, regulating neuronal excitability. This action stabilizes the resting membrane potential, a pivotal factor in mitigating the hyperexcitability that characterizes epilepsy.
Ezogabine has gained approval as an adjunctive treatment...
434
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several...
9.6K
Non-gated Ion Channels01:24

Non-gated Ion Channels

Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
7.7K
Antiarrhythmic Drugs: Class I Agents as Sodium Channel Blockers01:22

Antiarrhythmic Drugs: Class I Agents as Sodium Channel Blockers

Class I antiarrhythmic drugs are used to treat various types of arrhythmias or irregular heart rhythms. These drugs block the sodium (Na+) channels in the cardiac cells, thereby affecting the movement of electrical impulses across the heart. Class I antiarrhythmic drugs are divided into three subgroups: Class IA, Class IB, and Class IC, each with distinct mechanisms of action and effects on the heart.
Class 1A Antiarrhythmic Drugs: These drugs work by moderately blocking sodium channels,...
2.3K
Regulation of Sodium and Potassium01:26

Regulation of Sodium and Potassium

The regulation of sodium and potassium ion concentrations in the human body is a complex process governed primarily by hormones such as aldosterone, antidiuretic hormone (ADH), and atrial natriuretic peptide (ANP).
Sodium Regulation
Sodium ions make up approximately 90% of extracellular cations, with a normal blood plasma concentration of 136–148 mEq/L. A decrease in blood volume and pressure triggers the release of renin from granular cells in the juxtaglomerular complex (JGC), primarily...
1.6K