Related Experiment Video
Updated: Nov 23, 2025
10:26
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
Published on: January 20, 2019
12.7K
USP21 promotes cell proliferation by maintaining the EZH2 level in diffuse large B-cell lymphoma
Huixian Ma1, Xiangrui Luo1, Peng Zhou1
1Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, College of Life Sciences, Institute of Biomedical Sciences, Shandong Normal University, Jinan, China.
Journal of Clinical Laboratory Analysis
|January 3, 2021
Summary
Ubiquitin-specific peptidase 21 (USP21) promotes diffuse large B-cell lymphoma (DLBCL) cell proliferation by stabilizing enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2). This finding clarifies a key molecular mechanism in DLBCL development.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Diffuse large B-cell lymphoma (DLBCL) is the most prevalent form of non-Hodgkin lymphoma (NHL).
- The precise molecular mechanisms driving DLBCL pathogenesis are not fully understood.
Purpose of the Study:
- To investigate the role of ubiquitin-specific peptidase 21 (USP21) in DLBCL.
- To elucidate the molecular mechanisms by which USP21 influences DLBCL cell behavior.
Main Methods:
- Real-time PCR and Western blot were used to measure USP21 and EZH2 expression.
- CCK8 assays and cell death staining assessed USP21's impact on proliferation and apoptosis.
- The catalytic site (cysteine 221) of USP21 was examined for its role in proliferation regulation.
Main Results:
- USP21 expression was elevated in DLBCL lymphoid tissue.
- USP21 significantly enhanced DLBCL cell proliferation but did not affect cell death.
- USP21 stabilizes enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2), a protein crucial for lymphoma formation.
Conclusions:
- USP21, a deubiquitinase, drives DLBCL cell proliferation.
- USP21 maintains EZH2 protein levels, thereby promoting DLBCL progression.
More Related Videos
Related Concept Videos
02:23Abnormal Proliferation
4.9K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.9K
01:23Negative Regulator Molecules
37.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
37.5K
02:34Inhibition of Cdk Activity
5.3K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.3K
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.4K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.4K
01:27Molecular Factors Affecting Cell Division
3.6K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.6K
01:14Maintenance of the ES Cell State
2.4K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.4K