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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
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[Evaluation of Conversion Factors for Genetically Modified Maize Quantification].

Reona Takabatake1, Mari Onishi2, Junichi Mano1

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Summary

Conversion factor (Cf) values are essential for quantifying genetically modified (GM) crops. This study determined crucial Cf values for GM maize using real-time PCR instruments, aiding accurate GMO quantification.

Keywords:
conversion factorgenetically modifiedmaizequantitative methodreal time PCR

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Area of Science:

  • Agricultural Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Accurate quantification of genetically modified (GM) crops like maize and soybean relies on conversion factor (Cf) values.
  • These factors convert DNA copy number ratios to weight-based amounts, crucial for regulatory compliance.
  • Existing Cf values are available for GM soybeans on various real-time PCR instruments, but are lacking for GM maize.

Purpose of the Study:

  • To determine and provide essential conversion factor (Cf) values for the accurate quantification of genetically modified (GM) maize.
  • To address the current gap in Cf values for GM maize across multiple real-time PCR platforms.

Main Methods:

  • Experimentally determined conversion factor (Cf) values for GM maize.
  • Utilized four real-time PCR instruments: QuantStudio5, QuantStudio12K Flex, LightCycler 96, and LightCycler 480.
  • Targeted specific genetic sequences including Cauliflower mosaic virus 35S promoter (P35S), GA21, MIR604, and MIR162.

Main Results:

  • Successfully determined conversion factor (Cf) values for GM maize quantification.
  • Generated data applicable to the specified real-time PCR instruments.
  • Provided critical data for event-specific and promoter-specific GM maize detection.

Conclusions:

  • The determined conversion factor (Cf) values enable accurate weight-based quantification of GM maize.
  • This research fills a significant data gap, supporting regulatory and quality control processes for GM maize.
  • The findings facilitate reliable GMO analysis across different real-time PCR platforms.