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MicroRNA 452 regulates ASB8, NOL8, and CDR2 expression in colorectal cancer cells

Ji-Su Mo1,2, Soo-Cheon Chae3,4

  • 1Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, 54538, Republic of Korea.

Genes & Genomics
|January 5, 2021
PubMed
Abstract

Insights

MicroRNA 452 (MIR452) targets ASB8, NOL8, and CDR2 in colorectal cancer (CRC). Upregulated MIR452 in CRC downregulates these genes, suggesting their role in colorectal carcinogenesis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs are crucial in disease pathogenesis by regulating gene expression.
  • MicroRNA 452 (MIR452) is upregulated in early-stage human colorectal cancer (CRC).

Purpose of the Study:

  • To identify and validate MIR452 target genes implicated in CRC.
  • To investigate the regulatory role of MIR452 in colorectal carcinogenesis.

Main Methods:

  • Utilized a luciferase reporter system to confirm MIR452's effect on ASB8, NOL8, and CDR2.
  • Employed quantitative RT-PCR (qRT-PCR) and western blotting to assess gene and protein expression levels.

Main Results:

  • Confirmed MIR452 directly targets ASB8, NOL8, and CDR2.
  • Demonstrated that upregulated MIR452 downregulates ASB8, NOL8, and CDR2 mRNA and protein in CRC cells.
  • Observed decreased CDR2 protein expression in CRC tissues compared to non-tumor tissues.

Conclusions:

  • ASB8, NOL8, and CDR2 are validated target genes of MIR452 in CRC.
  • Upregulated MIR452 contributes to colorectal carcinogenesis by regulating ASB8, NOL8, and CDR2 expression.
Keywords:
ASB8CDR2Colorectal cancerMIR452NOL8

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