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LncRNA ROR1-AS1 accelerates osteosarcoma invasion and proliferation through modulating miR-504
Xiangkun Wu1, Lihua Yan2, Yongxi Liu1
1Department of Orthopaedic Surgery, Nanyang Second People's Hospital, Nanyang 473000, Henan, China.
Aging
|January 5, 2021
Summary
Long non-coding RNA ROR1-AS1 promotes osteosarcoma growth and migration by sponging miR-504. This suggests ROR1-AS1 acts as an oncogene and a potential therapeutic target for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (LncRNAs) are implicated in tumor development.
- The specific role of ROR1-AS1 in osteosarcoma remains uncharacterized.
Purpose of the Study:
- To investigate the functional role of ROR1-AS1 in osteosarcoma progression.
- To explore the underlying molecular mechanisms of ROR1-AS1 in osteosarcoma.
Main Methods:
- Quantitative real-time PCR to assess ROR1-AS1 expression.
- Cell proliferation, cell cycle, and migration assays in MG-63 cells.
- Dual-luciferase reporter assay to confirm the interaction between ROR1-AS1 and miR-504.
Main Results:
- ROR1-AS1 was upregulated in osteosarcoma tissues and promoted cell proliferation, cell cycle progression, and migration.
- ROR1-AS1 overexpression increased cyclin D1, PCNA, ki-67, N-cadherin, and vimentin, while decreasing E-cadherin.
- ROR1-AS1 acted as a molecular sponge for miR-504, and miR-504 overexpression partially reversed ROR1-AS1-induced effects.
Conclusions:
- ROR1-AS1 functions as an oncogene in osteosarcoma.
- ROR1-AS1 may serve as a novel therapeutic target for osteosarcoma treatment.
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