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TEMPy2: a Python library with improved 3D electron microscopy density-fitting and validation workflows
Tristan Cragnolini1, Harpal Sahota1, Agnel Praveen Joseph1
1Institute of Structural and Molecular Biology, Birkbeck, University College London, London, United Kingdom.
Acta Crystallographica. Section D, Structural Biology
|January 6, 2021
Abstract
None:
Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.

