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Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans
Carol A Woolford1, Aaron P Mitchell2
1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA. cw2g@andrew.cmu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|January 6, 2021
Abstract:
Here we present a protocol of interest to those who want to look at the functional consequences of decreasing expression of essential genes constitutively as well as being able to study the regulatory pathways of that essential gene by also deleting one or two additional genes. This allows epistasis relationships to be determined.
