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Bioengineered Braided Micro-Nano (Multiscale) Fibrous Scaffolds for Tendon Reconstruction
Anjana Jayasree1, Shalumon Kottappally Thankappan2, Retheesh Ramachandran2
1Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Kochi 682 041, India.
ACS Biomaterials Science & Engineering
|January 6, 2021
Summary
This study developed a novel braided scaffold (mPCL-nCol-bFGF) that mimics native tendon tissue. The scaffold promotes tendon regeneration and reduces adhesion formation, showing promising results in animal models.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Peritendinous adhesion is a common complication after tendon injury, hindering natural healing.
- Mimicking native tendon's hierarchical structure is crucial for effective tendon tissue engineering.
Purpose of the Study:
- To fabricate a multiscale fibrous scaffold (mPCL-nCol-bFGF) mimicking native tendon architecture.
- To evaluate the scaffold's potential for tendon regeneration and prevention of adhesions.
Main Methods:
- Fabrication of a braided scaffold with aligned PCL micro/collagen-bFGF nano fibers, coated with alginate.
- In vitro assessment of cell viability, attachment, and proliferation using rabbit tenocytes under static and dynamic conditions.
- In vivo evaluation in an Achilles tendon defect model.
Main Results:
- Sustained release of basic fibroblast growth factor (bFGF) for 20 days.
- Enhanced tenocyte proliferation and tenogenic marker expression with bFGF and dynamic stimulation.
- Demonstrated tendon tissue regeneration with aligned collagen morphology in vivo within 12 weeks.
Conclusions:
- The mPCL-nCol-bFGF scaffold effectively supports tenocyte growth and differentiation.
- Dynamic stimulation further enhances the scaffold's regenerative potential.
- The developed scaffold shows promise for treating Achilles tendon defects and preventing adhesions.
