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Modulation of Hypertrophic Scar Formation Using Amniotic Membrane/Electrospun Silk Fibroin Bilayer Membrane in a
Mazaher Gholipourmalekabadi, Sadjad Khosravimelal, Zeinab Nokhbedehghan
1Molecular Pharmacology Department, Memorial Sloan Kettering Cancer Center, New York, NY 10065 United States.
ACS Biomaterials Science & Engineering
|January 6, 2021
Summary
This study shows a new bilayered membrane effectively reduces hypertrophic scarring by decreasing collagen and increasing MMP1. This scar treatment offers potential for wound healing and tissue engineering.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Dermatology
Background:
- Hypertrophic scarring is a challenging dermal disorder caused by excessive extracellular matrix deposition.
- Current treatments for hypertrophic scars have limitations, necessitating novel therapeutic approaches.
- Decellularized human amniotic membrane and electrospun silk fibroin are promising biomaterials for tissue regeneration.
Purpose of the Study:
- To evaluate the efficacy of a 3D bilayered decellularized human amniotic membrane/electrospun silk fibroin membrane in minimizing hypertrophic scar formation.
- To assess the in vivo performance of the bilayered membrane in a rabbit ear model of hypertrophic scarring.
- To determine the impact of the bilayered membrane on collagen and MMP1 expression in scar tissue.
Main Methods:
- Fabrication of a 3D bilayered membrane using decellularized human amniotic membrane and electrospun silk fibroin.
- Immersion of the bilayered membrane in 70% ethanol to ensure layer adhesion.
- In vivo implantation of the bilayered membrane in a rabbit ear model to treat full-thickness hypertrophic scars.
- Assessment of scar characteristics, collagen deposition, and MMP1 expression at 28 and 50 days post-implantation.
Main Results:
- The bilayered membrane significantly reduced collagen deposition and expression in hypertrophic scars compared to controls and amniotic membrane alone.
- A significant increase in MMP1 expression and deposition was observed in wounds treated with the bilayered membrane.
- Histological analysis at days 28 and 50 post-implantation confirmed the antiscarring effects of the bilayered construct.
Conclusions:
- The fabricated 3D bilayered membrane demonstrates significant potential as an effective antiscarring wound dressing.
- This biomaterial construct may also be suitable for soft tissue engineering applications.
- The combination of amniotic membrane and silk fibroin offers a promising strategy for managing hypertrophic scarring.
