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Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
Published on: February 23, 2017
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Multifunctionalization Modulates Hydroxyapatite Surface Interaction with Bisphosphonate: Antiosteoporotic and
Lucia Forte1, Stéphanie Sarda2, Paola Torricelli3
1Department of Chemistry "G. Ciamician", University of Bologna, via Selmi 2, 40126 Bologna, Italy.
ACS Biomaterials Science & Engineering
|January 6, 2021
Summary
This study developed multifunctional biomaterials by combining hydroxyapatite, zinc, and polyethylenimine with risedronate. These novel materials show enhanced bone antiresorptive properties and protect osteoblasts from oxidative stress.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Bone Biology
Background:
- Osteoporosis is characterized by bone resorption, leading to fractures.
- Developing effective bone antiresorptive agents is crucial for osteoporosis treatment.
- Hydroxyapatite (HA) nanocrystals offer a promising scaffold for drug delivery.
Purpose of the Study:
- To create multifunctionalized biomaterials with enhanced bone antiresorptive properties.
- To investigate the adsorption and release mechanisms of risedronate on functionalized HA.
- To evaluate the efficacy of these composite materials in an in vitro model of oxidative stress-induced osteoporosis.
Main Methods:
- Synthesis of zinc-ion and polyethylenimine (PEI)-functionalized hydroxyapatite (HA) nanocrystals.
- Adsorption of bisphosphonate (risedronate) onto the functionalized HA.
- Characterization of material composition and adsorption isotherms (Langmuir and Freundlich models).
- In vitro cell culture studies using a coculture model of osteoblasts and osteoclasts under oxidative stress conditions (H2O2).
Main Results:
- The composite material (ZnHAPEIBP) showed approximately 8 atom % zinc incorporation and 5.9 wt % PEI content.
- Risedronate adsorption on ZnHAPEI followed a Freundlich model, indicating higher adsorption capacity compared to ZnHA (Langmuir model).
- The composite materials increased osteoblast viability and counteracted oxidative stress-induced impairment.
- Both PEI and risedronate reduced osteoclast viability and inhibited osteoclast activity.
Conclusions:
- Multifunctionalized biomaterials incorporating risedronate, zinc, and PEI on HA nanocrystals exhibit potent bone antiresorptive properties.
- These materials demonstrate a novel ability of risedronate to mitigate oxidative stress effects on osteoblasts.
- The developed composite materials show significant potential for osteoporosis treatment by enhancing bone formation and inhibiting bone resorption.
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