Systemically Administered Hemostatic Nanoparticles for Identification and Treatment of Internal Bleeding

Area of Science:

• Biomaterials Science
• Nanotechnology
• Medical Imaging

Background:

• Internal bleeding is challenging to diagnose and treat non-invasively.
• Current hemostatic nanoparticles lack diagnostic imaging capabilities.
• Need for integrated therapeutic and diagnostic solutions for internal hemorrhage.

Purpose of the Study:

• To develop polymeric nanoparticles for simultaneous treatment and imaging of internal bleeding.
• To incorporate multiple imaging tracers into hemostatic nanoparticles.
• To evaluate the efficacy of these multifunctional nanoparticles in a rodent model.

Main Methods:

• Synthesized polymeric nanoparticles incorporating a near-infrared (NIR) fluorescent dye (DiD), biotin, and gold nanoparticles.
• Tested nanoparticle hemostatic efficacy and biocompatibility.
• Utilized NIR imaging, immunohistochemistry, and X-ray computed tomography (CT) for nanoparticle visualization.
• Assessed nanoparticle accumulation at liver resection injury sites in rodents.
• Monitored survival rates post-injury.

Main Results:

• Developed multifunctional nanoparticles capable of hemostasis and multimodal imaging.
• Demonstrated successful visualization of nanoparticles at the liver injury site using NIR, immunohistochemistry, and CT.
• Observed highest nanoparticle accumulation at the bleeding site.
• Achieved significantly improved survival rates in rodents with liver resection injury.

Conclusions:

• Multifunctional nanoparticles offer a promising approach for simultaneous treatment and diagnosis of internal bleeding.
• Tracer incorporation enables visualization via standard clinical imaging modalities, aiding in localization.
• These advancements can improve emergency response and clinical management of internal hemorrhage.