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Related Experiment Video

Updated: Nov 22, 2025

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates
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Modular Chamber Assembled with Cell-Replicated Surface for Capture of Cancer Cells.

He Sun1, Lulu Han1, Liwei Yang1

  • 1Liaoning Key Laboratory of Molecular Recognition and Imaging, School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, Dalian, Liaoning 116023, P. R. China.

ACS Biomaterials Science & Engineering
|January 6, 2021
PubMed
Summary

We developed a modular circulating tumor cell (CTC) capture device, the CTC chamber, for efficient cancer cell isolation from blood. This adaptable system achieves high capture yield and purity, aiding in cancer diagnosis.

Keywords:
CTC chamberPDMScancer cellscapturecell-replicated surface

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Cell Biology

Background:

  • Circulating tumor cells (CTCs) are crucial biomarkers for cancer detection and monitoring.
  • Current CTC capture methods often involve complex microfluidics and limited sample volumes.

Purpose of the Study:

  • To develop a novel, modular CTC capture apparatus named the CTC chamber.
  • To enhance CTC capture efficiency and adaptability for varying blood sample volumes.

Main Methods:

  • The CTC chamber utilizes a modular design with polydimethylsiloxane (PDMS) films featuring topological structures and anti-EpCAM antibody coating.
  • The capacity is adjustable by altering the number of CTC-capture modules.
  • Experiments were conducted using spiked blood samples representing different cancer stages.

Main Results:

  • High capture yield (EpCAM-positive: ~80%, EpCAM-negative: ~65%) and purity (EpCAM-positive: ~90%, EpCAM-negative: ~80%) were achieved within 1 hour.
  • The modular design successfully accommodated different blood volumes (3 mL and 9 mL).

Conclusions:

  • The CTC chamber offers an economic and facile approach for CTC capture.
  • This technology has potential for next-generation CTC detection devices for various cancer stages.