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Related Experiment Video

Updated: Nov 22, 2025

Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
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Hand-Maneuverable Collagen Sheet with Micropatterns for 3D Modular Tissue Engineering.

Jaejung Son1, Min Seo Bang1, Je-Kyun Park1

  • 1Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

ACS Biomaterials Science & Engineering
|January 6, 2021
PubMed
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This study introduces a novel method using paper supports to easily manipulate thin collagen sheets with cells. This technique enables the creation of 3D endothelial networks for tissue engineering and drug testing applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Modular tissue engineering utilizes microscale units for complex 3D tissue reconstruction.
  • Hydrogel sheets offer advantages in assembling 3D microenvironments compared to microcapsules or microfibers.
  • Manipulating thin hydrogel sheets, especially those made of extracellular matrix (ECM) proteins like collagen, presents significant challenges.

Purpose of the Study:

  • To develop a novel fabrication technique for manipulating thin collagen sheets containing cells using a paper support.
  • To establish a sterile method that maintains high cell viability and intrinsic cell morphology.
  • To demonstrate the creation of functional endothelial modules with 3D tubular networks.

Main Methods:

  • A donut-shaped paper support was integrated with a micropatterned collagen sheet.
Keywords:
3D tissuecollagenhydrogel sheetmicropatternmodular tissue engineering

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Last Updated: Nov 22, 2025

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  • A cell-collagen mixture was permeated through a micropatterned mold into the paper support.
  • Sterile techniques were employed to ensure cell viability and morphology preservation.

    • Main Results:

    • A fabrication technique for thin collagen sheets with cells on a paper support was successfully established.
    • The method maintained high cell viability and intrinsic cell morphology.
    • An endothelial module exhibiting 3D tubular networks was demonstrated, with pattern length controlling tubular size and network density.

    Conclusions:

    • The developed technique facilitates the manipulation of collagen sheet modules for tissue engineering.
    • Geometrically controlled collagen sheet modules show potential for improved implantation applications.
    • These modules are promising for biologically relevant drug testing platforms.