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Updated: Nov 22, 2025

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
Silicon-rhodamine isothiocyanate for fluorescent labelling
Veselin Nasufović1, Patrick Then2, Fabian Dröge3
1Friedrich-Schiller-University, Institute of Organic Chemistry and Macromolecular Chemistry, Humboldtstr. 10, 07743 Jena, Germany. hd.arndt@uni-jena.de.
Abstract:
An efficient synthesis for silicon-rhodamines was developed, enabling the preparation and evaluation of silicon-rhodamine isothiocyanate (SITC) as a novel tool for facile fluorescent labeling. Ease of use in conjugation to amino groups, high stability and excellent photophysical properties are demonstrated. SITC-actin was found to be neutral to F-actin polymerization induction and well suited for high resolution fluorescence microscopy.

