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[Elaboration of methodology for simultaneous morphological and physiological study of an isolated autonomic neuron
Abstract:
Methodical difficulties encountered at simultaneous physiologic and morphologic investigation of an isolated vegetative neuron with a preserved synaptic apparatus and studied under large magnification are discussed in the article. To carry out such investigations, the author suggests two models of morpho-physiological devices and a preparation of the frog isolated vegetative neuron with a preserved synaptic apparatus, which help to solve the following problems: an increase of working distance for the microelectrode; fixation of the object studied; application of physical contrasting techniques.